(b) Fluorescence emission spectra of BSA-Au nanocomplexes in different concentrations of BSA solution (λ ex = 470 nm). For further biomedical applications of BSA-Au nanocomplexes, cytotoxicity assessment on cells is essential to evaluate the potential. MTT assay buy AZD6738 was employed to investigate the cell viability of MGC803 cells incubated with different concentrations of BSA-Au nanocomplexes. Figure 5a shows that
negligible cell death and physiological state change of MGC803 cells were observed, even if treated with the highest dosage (50 μg/mL) of BSA-Au nanocomplexes. Data obtained from MTT assay indicated no cytotoxicity of BSA-Au nanocomplexes in the concentration range of 0~50 μg/mL, cell viability are more than 95% in comparison with control group (Figure 5b). These results indicated that BSA-Au nanocomplexes possessed non-cytotoxicity and excellent biocompatibility on MGC803 cells within 0~50 μg/mL. Figure 5 Cytotoxicity of BSA-Au nanocomplexes on MGC803 cells. (a) Morphology of MGC803 cells incubated with 50 μg/mL of BSA-Au nanocomplexes for 24 h at 37°C. (b) Dark toxicity of BSA-Au nanocomplexes to MGC803 cells incubated with 0~50 μg/mL of nanocomplexes for 24 h at 37°C. Cell viability was determined by
MTT assay. Data represents mean ± SD (n = 5). BSA, a ubiquitous plasma protein with a molecular weight of 66,500 Da, is composed of 580 amino acid residues [23, www.selleckchem.com/products/17-DMAG,Hydrochloride-Salt.html 24]. Due to their wide hydrophobic, hydrophilic, anionic, and cationic properties, BSA has been extensively used as a model protein in many fields including drug delivery , biomimetic mineralization , nanomaterial synthesis [27, 28], surface modification and intermolecular interaction , etc. More recently, our group has successfully prepared a series of semiconductor chalcogenides with different sizes and morphologies in a solution of BSA at room temperature [10, 27, 30]. In this case, BSA plays multifunctional roles: (1) to direct
the synthesis of Au nanocomplexes, (2) to stabilize the Au nanocomplexes, (3) to improve the biocompatibility of Au nanocomplexes, see more and (4) to provide bioactive functionalities into these nanocomplexes for further biological interactions or coupling. An appropriate use of such nanocomplexes for biological labeling requires the decoration of biomarker selleck kinase inhibitor molecules on the nanocomplexes’ surface [31, 32]. Folic acid (FA) molecules, actively targeting the folate receptors of cancer cells, were selected as a model and conjugated with BSA-Au-NH2 using a modification of the standard EDC-NHS reaction as described by Jönsson [33–35]. To determine the intracellular uptake and the targeting ability of BSA-Au-FA, dark-field scattering and fluorescence imaging were performed on MGC803 cells (Figure 6).