2003, Papatheodorou et al. 2006, Zhou et al. 2007). The original data suggested that DP, NO3-N, T and PO4-P were almost normally distributed, whereas the other parameters were positively skewed, with kurtosis coefficients significantly greater than three (95% confidence). After log-transformation

of these other parameters ( Kowalkowski et al. 2006, Zhou et al. 2007), all skewness and kurtosis values (except Chl a) were sharply reduced, ranging Bleomycin ic50 from –0.7742 to 0.5822 and from –0.7641 to 0.5840, which were less than the critical values. For CA and PCA, all parameters were also z-scale standardized to minimize the effects of differences in measurement units and variance and to render the data dimensionless ( Wu & Wang 2007, Zhou et al. 2007). CA produced a dendrogram with two groups at (Dlink/Dmax) × 100 < 300 (Figure 3). Group A consisted of stations 5, 7, 8, 13–17, 20–28, which is called the low nutrient group, and group B contained stations 1–4, 6, 9–12, 18, 19 and 29–32, called the high nutrient

group. The classifications varied significantly, because the stations in these groups had similar features (low or high nutrient concentration), although these are caused by different natural backgrounds. The stations of the low nutrient group were far away from the mainland or the upwelling areas, whereas the stations of the high nutrient group came from the Pearl River Estuary (stations 1, 2, 3, 32, 31), or the Everolimus mouse upwelling regions (stations 4, 6, 9, 10, 11, 12 from the north-east of the PIS; 29, 30 from the upwelling region in the

west of the PIS). Station 23 from the perennial cold cyclonic eddy region should be in the high nutrient group, but is in fact in the low nutrient group, since the upwelling driven by the perennial cold cyclonic eddy is not powerful enough at the surface (Wu 1991, Liao et al. 2006). Bartlett’s PI-1840 sphericity test was performed on the parameter correlation matrix to examine the validity of the PCA (Wu & Wang 2007, Zhou et al. 2007). The significant level of Bartlett’s sphericity test is 0 (p < 0.05), indicating that PCA may be useful in providing significant reductions in dimensionality. PCA was conducted on standardized data sets of Data1 (z-scale standardized with mean and variance of zero and one, respectively) to analyse the source identification of nutrients (Mendiguchía et al. 2007, Zhou et al. 2007) and find the best indicator for upwelling formation. The linear correlation coefficients between the variables are shown in Table 1. As we expected, dissolved oxygen was strongly positively correlated with Chl a, which is a natural process because marine phytoplankton are the major oxygen producers here ( Xu & Zhu 1999, Wu & Wang 2007). Table 2 summarizes the PCA results comprising the loadings and eigenvalues. According to the eigenvalue-one criterion, the first five PCs with eigenvalues > 1 were considered essential. They explained 78.65% of the total variance. According to Table 2, the main contribution to PC1, explaining 27.