46(95%CI 0.06-0.85). CONCLUSIONS: Fibroscan® and CAP™ were feasible

in most of NAFLD patients studied. However, the agreement Small Molecule Compound Library of these noninvasive methods when compared to liver histology was unsatisfactory. Besides the prevalence of high BMI, perhaps the heterogeneous fibrosis and steatosis of NAFLD liver histology were to blame. Disclosures: The following people have nothing to disclose: Denise S. Vanni, Claudia P. Oliveira, Daniel F. Mazo, Fabiola Rabelo, José Tadeu Stefano, Flair J. Carrilho Introduction: Nonalcoholic steatohepatitis (NASH) has been associated with low levels of hepatic polyunsaturated fatty acids (PUFA), particularly omega-3 PUFA. There is no data on whether omega-3 PUFA modulate microRNA (miRNA) expression in liver. Some studies have reported altered miRNA expression in obese patients with NASH but there are no studies on whether there is an association with PUFA. The aim of this study was 1) to compare miRNA expression between patients with simple steatosis (SS), NASH, and healthy controls (HC), and 2) to examine correlations between

hepatic miRNA expression and omega-3 PUFA. Methods: miRNA expression was measured by NanoString technology in liver tissue from 24 living liver donors as healthy controls (HC; n= 24) and patients with biopsy proven SS (n= 25) or NASH (n= 22). Groups were compared by t-test ( p<0.001). Polyunsaturated fatty acids in hepatic http://www.selleckchem.com/products/AZD2281(Olaparib).html total lipids were measured by gas chromatography. Results are given in % of total fatty acids. Spearman correlations were used to identify potential associations. Results: Twenty-six miRNAs were differentially expressed between HC, SS and NASH, including

miR1 0b which was upregulated in HC vs NASH (p=0.00001). Total omega-3 PUFA were lower in NASH (mean± SD) (2.35±0.65 %) and SS (3.28±1.23 %) compared with HC (4.44±1.61 %) (p<0.05). Docosahexaenoic acid (DHA) and eicosapentaenoic acid (EPA), the biologically active long-chain omega-3 PUFA, were also lower in NASH and SS than in HC (p<0.05). Twenty of the differentially expressed miRNAs were significantly correlated with at least one omega-3 PUFA, including miR1 0b which was positively correlated with DHA (r=0.417, p=0.001) and total omega-3 PUFA (r=0.343, p=0.008). Conclusion: The 上海皓元医药股份有限公司 expression of miR1 0b was higher in HC than NASH and positively correlated with hepatic omega-3 PUFA. A potential target of miR1 0b is peroxisome proliferator-activated receptor-α, which can contribute to steatogenesis and inflammation in NAFLD. These results support the concept of associations between PUFA, epi-genetic mechanisms, and NAFLD-related gene expression. Further studies are required to establish cause-effect relationships and examine the potential of omega-3 PUFA supplementation to regulate miRNA in NAFLD. Disclosures: David W.