A mixture of the sensitivity of the B camera and the accuracy with which the microfluidic process controls the microenvironment allows for radioassays of the single-cell culture. 18F FDG uptake per cell for both M229 and M202 cancer cell lines was consistent for cell populations including 200 cells down to just one cell when incubated with a radioactivity focus of 37 MBq/mL throughout the radiotracer incubation time. Melanomas can have 1 of 3 driver oncogenic events in the mitogen activated protein kinase pathway: equipment mutations, N Ras mutations, ubiquitin conjugation and T Raf mutations. These are mutually exclusive strains, suggesting a dominant oncogenic event in the development of a likely therapeutic goal and this cancer. As a means to test whether the B camera and microfluidic chip might be used to evaluate differential therapeutic activity we took advantage of the specific anti-tumor effects of a book N Raf inhibitor, PLX4032, in cancer cell lines with defined oncogenic mutations. M229 features a homozygous BRafV600E mutation and is highly sensitive to PLX4032, having a 50% inhibition concentration of 0. 2 uM, whereas M233 has a heterozygous B RafV600E mutation but is resistant for this therapy, using a 50% inhibition concentration Chromoblastomycosis of more than 10 uM. M202 features a mutually exclusive Deborah Ras Q61 R mutation, and M257 is wild type for both D and BRaf Ras, with both cell lines also being immune to PLX4032. Macroscopic radioassays were also done as an easy way to confirm and assess the microfluidic results showing a decline in 18F FDG uptake of M229 cells treated with 1 uM PLX4032. There are several differences in method involving the microfluidic and macroscale techniques. In contrast to the macroscopic well dish studies, in each chamber, a smaller citizenry of cells was cultured. Therefore, a higher radioactivity concentration was combined with the ATP-competitive Chk inhibitor B camera studies, to improve the total sign available from each sample. In addition, the limited level of each microfluidic chamber also required that cell method be replenished every 6 h through the microfluidic radioassay. A bonus of the microfluidic system is that it can provide a method for preserving cell cultures for long periods in an environment by which perturbations can be precisely controlled. In comparison, macroscopic studies may do just a single radioassay on a given cell culture test because each description is an end-point study demanding that the cells be disturbed or removed from the culture environment. Compared with traditional macroscopic radioassays, that offer high sensitivity for radioactive detection using large samples, microfluidic processor and the B camera provide electronic control of small populations of cell cultures and the capacity to conduct radioassays of live cells in real-time and in vitro.