As one possible option for defining the dose addition relationship of odorants, we introduced a new testing approach in which relationships established between single therefore odorants are used for those of mixtures by means of the dilution-to-threshold (D/T) ratios derived by the air dilution sensory (ADS) test [9]. Based on this approach, we were able to show that the competing relationships hold between different odorants contained in the mixture of H2S and many carbonyls and that the patterns of masking phenomena change with their concentrations. In order to continue our efforts to investigate the odor mixing phenomenon, our testing approach for the dose addition relationship was employed to investigate the mixture of four reduced sulfur compounds (RSCs).

Based on these experiments, we Inhibitors,Modulators,Libraries discuss how the effect of mixing occurs Inhibitors,Modulators,Libraries between the odorants of the same family (or similar chemical properties). The results of our initial research on the effects of mixing between different odorant families (i.e., RSCs and aldehydes) have been reported elsewhere [3].2.?Materials and Methods2.1. Experimental Scheme for Masking EffectsIn this study, the effects of synthetic mixing were investigated using four individual RSCs consisting of hydrogen sulfide (H2S), methanethiol (CH3SH), dimethylsulfide (DMS), and dimethyldisulfide (DMDS). These compounds were selected because of their significance as the key offensive odorant group designated by the malodor prevention law of Korea [10]. The environmental impact of RSC in terms of malodor components released by industrial activities Inhibitors,Modulators,Libraries has been described in a number of previous studies [11�C13].

The basic experimental scheme of our study is presented in Figure 1. The major components of this study can be divided into two stages. Inhibitors,Modulators,Libraries In the first stage, the relationships between different expression units for odor composition [e.g., Entinostat odor concentration vs. odor intensity (OI)] are defined based on the pre-existing definition of their interactions [14,15]. More specifically, OI is first calculated from the known concentrations of each RSC whose samples are prepared to represent 11 OI ratings (Table 1). These individual samples are then subject to the ADS test to assign the corresponding D/T ratios to each individual sample.

After all, by binding all these concepts (concentration, OI, and D/T ratios), one can eventually establish selleck chemical empirical relationships between the odorant concentration and the D/T ratios, which were not initially linked. At the next stage, the newly established equations from the stage 1 experiment is then used to estimate the D/T ratios of the four RSC mixture (M4) samples in relation to each individual component. Hence, information concerning these estimated D/T results in stage 1 is used to assess the contributions of individual RSC components to the overall strengths of odor mixture in stage 2.Figure 1.