As the concentration increases, the value of T/C reaches the capacity. The device realized quantitative detection with a sensitivity of 20 pg/mL. Figure 9 Graph of T/C in different concentrations. Conclusions In conclusion, a CCD-based
reader was designed and fabricated, the quantitative analysis software was selleck compound compiled, and the resultant CCD-based reader system was used for quantitative analysis of examined CagA antigen on the strips. A fluorescence detection system of lateral flow strip was developed. A revised WTHE algorithm was used to enhance captured QD test strip images. Practical results indicated that the system could quickly and accurately detect the fluorescence signal. QD lateral flow tests were used with different concentrations SAHA HDAC price to detect CagA samples and indicated that the sensitivity of this device was 20 pg/mL. For a future study, test strips with multilines could be detected and some wireless technologies could also be applied in similar instruments. More nanoparticles could be applied for improving sensitivity, which is also a big issue. Authors’ information DC is a professor of Shanghai Jiao Tong University. His research interests include the synthesis of nanomaterials and their application in the biomedical field. KW is a lecturer of Shanghai Jiao Tong University. Her scientific interests are nanotechnology development of CYC202 purchase early cancer detection
and screening equipment, nonmaterial molecular imaging, and biocompatibility evaluation. CL is a PhD candidate of Shanghai Jiao Tong University. XD and CG are both master students of Shanghai Jiao Tong University. Acknowledgements We are grateful for the financial support by the Chinese 973 Project (2010CB933902 and 2011CB933100), National Natural Science Foundation of China (No.81101169,
81225010, and 81327002), Shanghai Science and Technology Fund (13 nm1401500 and 11 nm0504200), Important National Science and Technology Specific Projects(2009ZX10004-311), and 863 High-Tech Project of China (2012AA0022703). References 1. Mei JC, Ye Ixazomib Q, Zhou WY: Development and study of lateral flow test strip reader based on embedded system. In 2011 10th International Conference on Electronic Measurement &Instruments (ICEMI): 16–19 Aug 2011; Chengdu. Piscataway: IEEE; 2011:201–204. 2. Huang LH, Zhou L, Zhang YB: A simple optical reader for upconverting phosphor particles captured on lateral flow strip. Sensors Journal, IEEE 2009, 9:1185–1191.CrossRef 3. Shyu RH, Shyu HF, Liu HW: Colloidal gold-based immunochromatographic assay for detection of ricin. Toxicon 2002, 40:255–258.CrossRef 4. Liu G, Lin YY, Wang J: Disposable electrochemical immunosensor diagnosis device based on nanoparticle probe and immunochromatographic strip. Anal Chem 2007, 79:7644–7653.CrossRef 5. Li Z, Wang Y, Wang J: Rapid and sensitive detection of protein biomarker using a portable fluorescence biosensor based on quantum dots and a lateral flow test strip.