Epochs including artifacts HSP inhibitor drugs such as eye blinks and eye movements identified by visual

inspection were excluded from the analyses. To identify the sources of the evoked activities, ECD analyses were performed using commercial software (MEG 160; Yokogawa Electric Corporation). The ECDs with goodness of fit (GOF) values above 90% were used, based on a previous report (Bowyer et al., 2003, Dalal et al., 2008 and Sekihara and Nagarajan, 2008). Anatomical magnetic resonance imaging (MRI) was performed using a Philips Achieva 3.0TX (Royal Philips Electronics, Eindhoven, the Netherlands) to permit registration of magnetic source locations with their respective anatomical locations. Before MRI scanning, five adhesive markers (Medtronic Surgical Navigation Technologies Inc., Broomfield, CO) were attached to the skin of their head (the first and second ones were located at 10 mm in front of the left and right tragus, the third

at 35 mm above the nasion, and the fourth and fifth at 40 mm right and left of the third one). The MEG data were superimposed on MR images using information obtained from these markers and MEG localization coils. The PFS is OSI-744 datasheet a questionnaire comprised of 15 items scored on a 5-point Likert-type scale ranging from 1 (Do not agree at all) to 5 (Strongly agree), with higher scores indicating greater responsiveness to food environment ( Lowe et al., 2009). Based on previous factor analyses, the PFS has been shown

to contain a three-factor structure of food proximity consisting of: (1) ‘food available’, which describes the reaction when food is not physically present but is always available; (2) ‘food present’, which characterizes the reactions to palatable foods when they are physically present, but have not yet been tasted; and (3) ‘food tasted’, which characterizes the reactions to palatable foods when first tasted, but not yet consumed. According to previous studies using the PFS ( Yoshikawa et al., 2012, Cappelleri et al., 2009 and Schultes et al., 2010), the subscale scores for PFS are calculated as the average scores of all items included in each subscale (ranged 1–5) as well Metalloexopeptidase as aggregated factor scores as the average scores of all 15 items (ranged 1–5). The participants completed the PFS before the MEG recordings. Data are expressed as mean±SD unless otherwise stated. Subjective levels of appetitive motives during the MEG recordings were compared between the Fasting and ‘Hara-Hachibu’ condition by paired-t test. All the MEG variables under four conditions (food images in the Fasting condition, mosaic images in the Fasting condition, food images in the ‘Hara-Hachibu’ condition, and mosaic images in the ‘Hara-Hachibu’ condition) were compared using two-way ANOVA for repeated measures. A paired t-test was used to evaluate significant differences between the two conditions.