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38. Cannata JJ, Cazzulo JJ: Glycosomal and mitochondrial malate dehydrogenases in epimastigotes of Trypanosoma cruzi. Mol Biochem Parasitol 1984, 11:37–49.CrossRefPubMed 39. Cazzulo JJ, Cazzulo Franke MC, Franke de Cazzulo BM: On the regulatory properties of the pyruvate kinase from Trypanosoma cruzi epimastigotes. FEMS Microbiol Lett 1989,50(3):259–263.CrossRefPubMed Authors’ contributions MAD designed the experiments, set up the techniques, 17-DMAG (Alvespimycin) HCl performed the experimental approaches, data analysis and interpretation and writing of the manuscript. LPD and BD performed the first

purification of the Tc38 antibody and collaborated in the assessment of Tc38 expression through life cycle by western blot. Digitonin solubilization and subcellular fractionation was performed by MAD and DM at Dr. J.J. Cazzulo’s lab. LP assisted with the immunofluorescence assays. JRSS did the confocal analysis and provide helpful guidance to set up Tc38 immunohistochemistry protocol. Immunofluorescence analysis of Tc38 in the life cycle stages as well as during cell cycle in asynchronic cultures of epimastigotes was done by LP and SN at SS’s lab. SS, BD and NW participated in helpful discussions of the results and critical reading of the manuscript. NW also collaborated in outlining the experimental strategies. BG conceived and designed the project, mentored MAD and reviewed the manuscript for its publication. All authors read and approved the final manuscript.”
“Background Aspergillus fumigatus (A. fumigatus) is a saprophytic mould that is responsible for life-threatening invasive pulmonary diseases in immunocompromised hosts.