(Obstet Gynecol 2011; 117: 452-5) DOI: 10.1097/AOG.0b013e3181f7381f”
“Jatropha curcas a member of the Euphorbiaceae family is a drought resistant small tree of great economic importance in developing countries because of its several industrial and medicinal values. The antioxidative properties, total phenolic content and DNA protection potential of raw and processed kernel meal extracts of Jatropha were analyzed. The samples were extracted with 70% acetone and used for the evaluation of its antioxidant potential. The total phenolic content of raw and processed Jatropha kernel buy WH-4-023 meal samples ranged between 9.06

and 22.22 mg TAN/g extract. The results of the study revealed that autoclaving and dry heat treatment causes a significant (P < 0.05) increases in the total phenolic content and antioxidant properties. Nevertheless, raw samples exhibited a high metal chelating activity (0.97 mg EDTA/g extract) compared to the processed samples. However, autoclaving with NaHCO3 and ash solution recorded the highest phenolic content (22.22 mg TAN/g extract), FRAP (2861.11 mmoL Fe(II)/g extract), phosphomolybdenum (353.61 click here mu moL ascorbic acid/g extract), DPPH (6.29g extract/g DPPH), nitric oxide (55%), superoxide (67%) and hydroxyl radical (49%) scavenging activity when compared to the raw and other processed samples. The present studies may be of importance in varietal

MI-503 nmr improvement, nutraceuticals and bio-pharmaceuticals as possible sources of cost-effective natural antioxidants. (C) 2012 Elsevier B.V. All rights reserved.”
“Mitosis is known to be regulated by protein kinases, including MPF, Plk1, Aurora kinases, and so on, which become active in M-phase and phosphorylate a wide range of substrates to control multiple aspects of mitotic entry, progression, and exit. Mechanistic investigations of these kinases not only provide key insights into cell cycle regulation, but also hold great promise for cancer therapy. Recent studies,

largely in Xenopus, characterized a new mitotic kinase named Greatwall (Gwl) that plays essential roles in both mitotic entry and maintenance. In this study, we generated a panel of mouse monoclonal antibodies (MAbs) specific for Xenopus Gwl and characterized these antibodies for their utility in immunoblotting, immunoprecipitation, and immunodepletion in Xenopus egg extracts. Importantly, we generated an MAb that is capable of neutralizing endogenous Gwl. The addition of this antibody into M-phase extracts results in loss of mitotic phosphorylation of Gwl, Plk1, and Cdk1 substrates. These results illustrate a new tool to study loss-of-function of Gwl, and support its essential role in mitosis. Finally, we demonstrated the usefulness of the MAb against human Gwl/MASTL.”
“Background and Purpose: Surgery is a high-stakes “”performance.