Statstcal analyss The statstcal sgnfcance in the dfference betweegroups for your vtro and vvo westerresults was determned by a single way ANOVA usng Bonferonns correctofor the multple posthoc analyses.Parametrc statstcs usng the general lnear model for repeated measures have been utilised to dentfy sgnfcant results of treatment condtons othe behavoral analyss of motor functon.The statstcal sgnfcance in the dfference betweegroups to the neurte length measurements was determned usng the College students check.All outcomes were expressed as meaSEM wth 0.05 consdered sgnfcant.Characterzatoof the EPO expressnghSvector vEPO Spnal cord neurons vtro transfected wth vEPO showed expressoofhA tagged EPO protecell lysate detected by Westerblot at 72hours post nfectousng anthA antbody to detect only the transgene.Release of EPO from the cells transfected wth vEPO at 48hrs following transfectowas demonstrated culture meda by Westerblot and quanttated by ELSA.No transgene EPO was detected underneath basal condtons or from cells transfected wth vC.
vvo, njectoof 2 uL of vEPO nto the cervcal spnal cord resulted robust EPO mRNA expressothat was sustaned for much more tha14 days.The expressoofhA tagged EPO protewas confrmed by Westerblot.ntraspnal njectoof vEPO decreases post traumatc cavty and nflammatory response SC njury was nduced supplier PS-341 by asymmetrc contusoothe rght dorsal spnal cord at C7 level.The degree of tssue njury and tssue sparng resultng from vector medated EPO expressowas determned by analyss ofh E staned seral sectons of spnal cord.Anmals handled wth vEPOhad sgnfcantly reduced post traumatc cavty area wththe spnal cord demonstratng decreased tssue harm as compared wth anmals taken care of wth vC.The spot of nflammatory nftrate was determned by mmunohstochemstry usng aant CD 45 monoclonal antbody.Anmals treated wth vEPOhad sgnfcantly reduced resdual location of post traumatc nflammatory selelck kinase inhibitor response as compared wth anmals handled wth vC.
Anmals handled wth vEPO show mproved forelmb motor functoafter SC Rght forelmb functowas assessed by modfed forelmb motor ratng

scale, forepaw slps the grdwalk, paw reachng and preference the cylnder check, and the paw placement check.Anmals njected ntraspnally vEPO 1h soon after SC showed a sgnfcantlyhgher forelmb ratng score, begnnng at two week just after njury and persstng over the course of 6 weeks publish njury in contrast wth the anmals njected wth control vector.A sgnfcant mprovement forelmb steppng accuracy the grd walk test whch was seealready at two weeks anmals treated wth vEPO compared to control, Both VEPO and vC treated mproved over 6 weeks of testng but vEPO treated anmals performed persstenly better that vC treated anmals.Analyss of paw reachng cylnder test was also markedly mproved and at 4, 5 and 6 weeks the forelmb performance was smar to sham anmals.