We screened a geographically diverse RepSox manufacturer panel of 132 European isolates belonging to the B.Br.013 group and a geographically diverse panel of 25 Georgian isolates across lineage-specific assays to determine whether they were in the B.Br.026 or the Georgian (B.Br.027) lineages (see additional file 3, Table 2). MLVA All 25 Georgian isolates were screened with an 11-marker MLVA system (Additional file 4) [25]. This was done in order to determine the level of genetic diversity within each identified subclade. The MLVA Diversity (D) was calculated for each subclade using the following equation: G/N (G = MLVA genotypes; N = number of isolates). Diversity was not calculated for

subclades with a single isolate. Authors’ information GC, MS, KU-57788 supplier National Center for Disease Control and Public Health, Tbilisi, Georgia DNB, PhD, Northern Arizona University, Flagstaff, Arizona MK, PhD, National Center for Disease Control and Public Health, Tbilisi, Georgia EZ, MS, National Center for Disease Control and Public Health, Tbilisi, Georgia GB, MS, National Center for Disease Control and Public Health, Tbilisi, Georgia NT, MD, Ph.D., National Center for Disease Control and Public Health, Tbilisi, Georgia ST, MD, Ph.D., National Center for Disease Control and Public Health, Tbilisi, Georgia PI, MD, Ph.D., National Center for Disease Control and Public Health, Tbilisi, Georgia

JF, Ph.D., U.S. Army Medical Research Institute of Infectious Diseases, Fort Detrick, Frederick, Maryland SMBS, PhD, Translational Genomics Research Institute, Phoenix, Arizona JSBS,

BS, Translational Genomics Research Institute, SCH727965 Phoenix, Arizona SS, MS, Translational Genomics Research Institute, Phoenix, Arizona MDC, PhD, Translational Genomics Research Institute, Flagstaff, Arizona MG, DVM, MSc, Veterinary Metalloexopeptidase Medical Research Institute, Hungarian Academy of Sciences, Budapest, Hungary AHP, Northern Arizona University, Flagstaff, Arizona ELK, Northern Arizona University, Flagstaff, Arizona JDB, PhD, Northern Arizona University, Flagstaff, Arizona TP, PhD, Northern Arizona University, Flagstaff, Arizona JTF, PhD, Northern Arizona University, Flagstaff, Arizona AJV, PhD, Northern Arizona University, Flagstaff, Arizona DMW, PhD, Northern Arizona University, Flagstaff, Arizona PK, PhD, Northern Arizona University, and Translational Genomics Research Institute, Flagstaff, Arizona Acknowledgements This work was funded by the U.S. Department of Homeland Security S&T CB Division Bioforensics R&D Program. Note that the use of products/names does not constitute endorsement by the Department of Homeland Security of the United States. Electronic supplementary material Additional file 1: Francisella tularensis canSNP revised SCHU S4 positions. Provides the updated SCHU S4 genome positions for Melt-MAMA assays published in Vogler et al. 2009. (XLS 20 KB) Additional file 2: Coverage plot of Illumina short sequence reads for Georgian strain F0673 aligned to LVS.