Skilled motor practice facilitates the formation of an internal model of movement, which may then be used to anticipate task-specific requirements at a later time (Shadmehr & Holcomb, 1997). Internal models are most susceptible to interference during and immediately following practice and become less susceptible to interference

over time through persistent neural activity, a process called consolidation (Brashers-Krug et al., 1996; Robertson et al., 2004). Motor memory consolidation can take place both explicitly, with conscious awareness, or implicitly, without conscious awareness of the skill being performed (Robertson et al., 2004). The neural processes of consolidation can take two forms: (i) online improvements that occur KU-60019 datasheet concurrent with practice or (ii) offline improvements that develop following the termination

of practice (Brashers-Krug et al., 1996; Robertson et al., 2004). Importantly, these two processes are not completely independent or mutually exclusive. Given its known role in the selection of movements (Kalaska & Crammond, 1995; Rushworth et al., 2003) and implicit motor learning (Ohbayashi et al., 2003; Meehan et al., 2011), the dorsal premotor cortex (PMd) is a logical candidate for involvement in motor memory consolidation. Our group reported improved implicit sequence-specific motor learning when 5 Hz repetitive BEZ235 mw transcranial magnetic stimulation (rTMS) was delivered over the PMd prior to skilled motor practice of a continuous tracking task (Boyd & Linsdell, 2009). Yet it is not clear whether improvements noted when PMd stimulation precedes motor practice result from only online or a combination of online and offline consolidation

of sequence-specific elements. The current work sought to directly triclocarban assess the involvement of PMd in offline consolidation of skilled motor practice. In contrast to our previous work (Boyd & Linsdell, 2009), three groups received either 1 Hz, 5 Hz or control rTMS immediately following practice of a continuous visuomotor tracking task (Experiment 1). Based on our previous work, it was hypothesized that 5 Hz rTMS immediately following practice would enhance while 1 Hz rTMS would suppress motor learning compared with control stimulation. However, the effects of TMS are known to be ‘state dependent’ (Silvanto et al., 2008). State-dependence has been demonstrated during both perceptual and cognitive tasks where prior or concurrent neural activity (Silvanto et al., 2007b; Arai et al., 2011) and/or task-specific elements (Bestmann et al., 2008; Cohen & Robertson, 2011) influence expected outcomes. An alternative hypothesis is that 1 Hz rTMS, typically associated with inhibition, over PMd immediately following practice may enhance implicit sequence-specific motor learning through state-dependent mechanisms.