0 −3.4 CPE2437 CPF_2747 (nrdH) glutaredoxin-like protein, YruB-family 3.8 −2.5 4.8 −11.0 CPE2551 CPF_2875 (glpA) probable glycerol-3-phosphate dehydrogenase 0.8 −2.5 1.3 −0.1 Purines, pyrimidines, nucleotides, and nucleosides CPE2276 CPF_2558 (guaB) inosine-5’-monophosphate dehydrogenase 9.2 −3.6 30.3 −1.5 CPE2622 CPF_2958 (purA) adenylosuccinate synthetase 4.3 −1.9 14.8 −0.8 Alvocidib mouse protein fate CPE0173 CPF_0166 (colA) collagenase 9.9 −4.7 8.5 −2.7 CPE2323 CPF_2632 (pepF) probable oligoendopeptidase F 2.7 -2.0 11.6 4.3 CPE1205 CPF_1002 (abgB)

amidohydrolase family protein 1.9 −4.3 67.4 buy RG7112 −1.6 Regulatory functions CPE0073 CPF_0069 transcription antiterminator 2.1 −5.0 1.9 −2.6 CPE0759 CPF_0753 putative regulatory protein 1.5 −5.4 3.3 0.6 CPE1533 CPF_1784 (scrR) sucrose operon repressor 1.7 −2.8 132 −1.5 CPE2035 CPF_2292 (hrcA) heat-inducible transcription repressor HrcA 2.3 −2.9 9.5 5.5 CPE2363 CPF_2673 two-component sensor histidine kinase 2.1 −3.0 16.1 2.7 Transport and binding proteins CPE1240 CPF_1450 (mgtE) magnesium transporter 8.6 −1.7 5.2 −2.6 CPE1300 CPF_1507 (gadC) glutamate:γ-aminobutyrate PI3K inhibitor antiporter family protein 9.6 −2.7 17.1 −7.3 CPE1505 CPF_1756 (uraA) uracil transporter 3.8 −2.7 3.9 −4.6 CPE0075 CPF_0070 N-acetyl glucosamine-specific 1.4 −14.3 1 .8 ND CPE0707 CPF_0703 ABC transporter, ATP-binding protein 1.5 −3.2 5.2 2.9 CPE0761 CPF_0756 (gltP) proton/sodium-glutamate symporter 1.5 −4.2

4.6 0.9 CPE1371 CPF_1621 sodium:neurotransmitter symporter family protein 1.8 −4.0 15.2 2.7 CPE2084 CPF_2341 (modB) molybdate

ABC transporter, permease protein 1.8 −2.5 10.8 2.0 CPE2343 CPF_2652 (malE) putative maltose/maltodextrin ABC transporter 2.9 1.3 3.8 −2.1 Unknown functions CPE0183 CPF_0176 nitroreductase family protein 1.0 −4.8 2.9 −1.1 CPE1172 CPF_1375 haloacid dehalogenase 2.1 −2.4 20.6 −1.7 CPE1784 CPF_2038 (nifU) NifU family protein 1.3 −2.5 6.4 −1.5 CPE2448 CPF_2758 PSP1 domain-containing protein 1.0 −2.4 5.5 −1.9 All of the data are the means of three different experiments. Table 2 Microarray analysis of the genes that were upregulated in one or both gatifloxacin-resistant mutants, 13124 R and NCTR R Gene ID and name Function/Similarity Microarray (mt/wt)       NCTR ATCC 13124 Amino acid biosynthesis     HSP90   CPE1520 CPF_1772 (ilvE) branched-chain amino acid aminotransferase 1.1 2.6 CPE1905 CPF_2161 (dapA) dihydrodipicolinate synthase 1.0 1.9 Cell envelope CPE0492 CPF_0465 capsular polysaccharide biosynthesis protein 6.5 1.9 CPE0495 CPF_0468 UDP-glucose/GDP-mannose dehydrogenase family 3.5 2.4 CPE2059 CPF_2316 putative membrane protein 7.1 3.2 CPE2079 CPF_2336 putative membrane protein 14.2 2.1 CPE0785 CPF_0787 putative membrane protein 2.3 2.1 Energy metabolism CPE2186 CPF_2451 (atpE) ATP synthase epsilon subunit 3.3 2.9 CPE2187 CPF_2452 (atpB) ATP synthase beta subunit 3.6 2.2 CPE2189 CPF_2454 (atpA) ATP synthase alpha subunit 4.2 2.4 CPE2190 CPF_2455 (atpH) ATP synthase delta subunit 1.9 2.