01 5.66 4.12 3.1 0.08 Rissani Kser Moulay Abdelleah Rissani 103-104 60 0 50 nt nt nt nt nt Rissani Mezguida Rissani 105-107 60 0 50 nt nt nt nt nt Selleckchem CH5183284 Errachidia Domaine Experimental Rich Errachidia 108-109 120 -5 45 nt nt nt nt nt Errachidia Aïne Zerka Rich Erracidia 110-117 120 -5 45 8.24 6.06 1.64 5.1 0.08 Aoufouss Ivacaftor Zaouit Amelkis Aoufouss 118 120 -5 40 nt nt nt nt nt Toudra Tinghir Tinghir 119-121 250 -0.5 42 8.1 5.12 2.07 9.4 0.04 Ziz Errachidia Ziz 122-129 130 0.5 42 nt nt nt nt nt
Ziz Erfoud Ziz 130-136 130 0.5 42 nt nt nt nt nt Rich Ziz Ziz 137-145 130 0.5 42 nt nt nt nt nt Chichaoua Mjjat Chichaoua 146 240 4.9 39 7.33 4.5 2.52 6.2 0.08 Alhaouz Asni Alhaouz 147-149 230 2 39 7.53 5.2 1.66 9.3 0.02 Tahanaout Tahanaoute 150-152 250 4 42 7.51 3.52 1.9 5.1 0.02 Alhaouz Tahanaout Imgdal Tahanaoute 153 250 4 42 7.23 6.09 1.9 5.1 0.02 Azilal Demnate Lahrouna Azilal 154-157 130 -1 42 7.73-8.21 5.89-5.97 1.75 4.5 0.02 ppm = mg/Kg soil aAverage data of 15 year as of year 2005 nt = Not tested Soil test interpretations (according to Rabusertib information available at ; ; Personal communication by Dr Abdelmajid Zouahri, INRA, CRRA, Rabat, Morocco): http://ag.arizona.edu/crops/cotton/soilmgt/saline_sodic_soil.html http://aces.nmsu.edu/pubs/_a/a-122.html
bFor EC: Saline soil = EC > 4 ds/m; Normal soil = EC < 4 ds/m c For Mn: low = <1.0 mg/Kg; moderate = 1.0-2.5 mg/Kg; high = >2.5 mg/Kg d For Zn: low = <0.5 mg/Kg; moderate = 0.5-1.0 mg/Kg; high = >1.0 mg/Kg e For Cd: all the soils samples above the normal level (0.01 mg/Kg of soil) The phenotypic characterization of the sampled 157 isolates for above characters revealed a large degree of variation much (Figure 2; Additional file 1). Figure 2 Growth of isolates under salinity (a), water stress (b), high temperature (c), under different pH (d); and their resistance to antibiotics. St: streptomycin; Cl: Chloramphenicol; Tr: Tetracycline;
Sc: Spectinomycin and Concentrations: 10, 15, 25, 50 and 100 μg/ml (e), and heavy metals (Mn 300 μg/ml; Zn, 200 μg/ml; Hg, 20 μg/ml and Cd 5 and 20 μg/ml) (f). Salinity is an important stress for rhizobia, because it inhibits persistence and development [17]. Consequently, a selection of rhizobia strains tolerant to salinity is of great importance for alfalfa cultivation in salt-affected areas. Indeed, after screening 157 isolates for salt tolerance, we observed a wide variability for tolerance at 171-1711 mM (1-10%) NaCl (Figure 2a); even isolates sampled from the same area/region showed variation for NaCl tolerance (compare Figure 3 and Table 2). 55.41% of the isolates (which includes 14 isolates of S. medicae) had good tolerance to NaCl (> 513 mM), indicating that the rhizobia nodulating alfalfa are more tolerant compared to other rhizobia species [3, 18].