2–)3 8–4 5(–5 0) × (3 0–)3 2–3 6(–4 5) μm, l/w (0 8–)1 1–1 3(–1 5

2–)3.8–4.5(–5.0) × (3.0–)3.2–3.6(–4.5) μm, l/w (0.8–)1.1–1.3(–1.5) (n = 100), subglobose or ellipsoidal; proximal cell (3.7–)4.3–5.5(–6.5) × (2.4–)2.5–3.2(–5.0) μm, l/w (0.7–)1.5–2.0(–2.5) (n = 100), wedge-shaped or oblong, less commonly subglobose. Anamorph on the natural substrate: gliocladium-like conidiophores to 250 μm long, with dry green

heads 30–100(–170) μm diam, appearing on or around stromata. Cultures and anamorph: optimal growth at 30°C on all media; good growth at 35°C. On CMD after 72 h 17–19 mm at 15°C, 51–58 mm at 25°C, 64–66 mm at 30°C, 48–53 mm at 35°C; mycelium covering the plate after 4 days at 25°C. Colony hyaline, thin; hyphae with conspicuous differences in width; mycelium mostly of primary hyphae, loose, forming radial strands; LY2874455 ic50 conspicuously wide (to ca 15 μm) at the marginal surface. Aerial hyphae absent or scant. Autolytic excretions lacking or rare, no coilings seen. No diffusing pigment, no distinct odour noted. Agar of cultures stored for ca 3 months at 15°C sometimes rosy. Chlamydospores noted after 1–2 days at 25–35°C, spreading from the centre across GSK461364 manufacturer entire plate, numerous, globose, mostly terminal in narrow hyphae. Conidiation noted after 2(–3) d at 25–35°C, green

after 3–4 days; effuse, first appearing mainly around the GSK126 price plug and along the margin as green to black dots 150 μm diam, growing to ca 0.5 mm diam, eventually arranged in indistinct concentric zones; zones becoming more distinct and regular with increasing temperature. Conidiophores (after 8 days) solitary or in fascicles of up to 10 to 0.6 mm wide in total; to 0.4 mm long including conidial head; originating from several hyphal fascicles (roots) and often surrounded by narrow hyphae on lower levels. Conidiophores consisting of a single erect, thick-walled stipe or main axis 7–13(–14) μm wide at the base, attenuated

to 7 μm upwards and mostly to 120 μm long to the first branching, smooth, appearing rough under low magnification due to guttules; repeatedly narrow branches growing out below septa, directed downwards, giving the impression of a MTMR9 synnema; bearing an apical penicillus of 3–4 levels of steeply ascending, nearly parallel unicellular branches originating on a single level, re-branching into whorls of (1–)4–5(–6) similar branches. Penicilli without conidial masses in mounts mostly to 100 μm long and 70–120 μm wide at the apex. Branches attenuated from 6 μm at the base to 2.5–3.5 μm upwards. Phialides formed densely appressed and parallel in whorls of 2–6 on terminal branches (=metulae) 2.5–3.5 μm wide. Phialides (6–)8–11(–12) × (1.8–)2.0–2.5(–3.0) μm, l/w (2.3–)3.4–5.1(–6.1), (1.0–)1.3–2.0(–3.0) μm wide at the base (n = 60), lageniform, subulate or subcylindrical, inaequilateral and curved when lateral in the whorl, neck short, becoming green with age.

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