45 mm and the scanning speed was 20 mm s-1. After chromatographic development, the bands were scanned in the range of 200�C400 nm (spectrum scan speed: 20 nm s-1) so that the drug could be estimated at 254 nm, which is ascertained by taking the spectrum at different concentrations between 100 and 500 ng with 100 ng increment. selleckchem Y-27632 Further, it is also observed that the spectra are similar in their behavior. Procedure for the standard The standard stock solution of mycophenolate mofetil was applied on a TLC plate, (1 ��L) by using the Linomat V sample applicator and the 100 ��l syringe. The plate was developed and scanned under the conditions described above. Each amount was analyzed five times and peak areas were recorded. A calibration plot of peak area against the respective amount was established for mycophenolate mofetil.

Procedure for the sample Twenty tablets were weighed accurately and finely powdered. A quantity of powder equivalent to 10 mg mycophenolate mofetil was weighed and transferred to a 100 mL volumetric flask containing approximately 50 mL methanol. The mixture was ultrasonicated for five minutes; then, the final dilution was made with methanol. The solution was filtered using Whatmann 41 paper, and 3 ��l of the filtrate was applied on a TLC plate. After the development of the chromatogram, the peak area of the bands was measured at 254 nm and the amount of drug in each tablet was determined from the calibration plot. The analytical procedure was repeated six times for the homogenous powder sample.

Method validation The limit of detection (LOD) and limit of quantitation (LOQ) for mycophenolate mofetil was calculated from the linearity data using relative standard deviation of the response and slope of the calibration curve for mycophenolate mofetil. The LOD of a compound is defined as the lowest concentration that can be detected. LOD value was found to be 35.33 ng/band for mycophenolate mofetil. LOQ is the lowest concentration of a compound that can be quantified with acceptable precision and accuracy. The LOQ value was found to be 120.72 ng/band for mycophenolate mofetil. To study intraday and interday precision, three different concentrations of sample solutions were prepared (100, 300, and 500 ng/ band) and applied to the TLC plates. All the solutions were analyzed in triplicate on the same day and on three different days to record intraday and interday variations in the results, respectively.

To check the accuracy of the method, recovery measurements were performed by the addition of the standard drug solution at three different levels (50, 100, and 150%) to the preanalyzed sample solution (200 ng/band for mycophenolate mofetil so that after Cilengitide the addition of standards, the samples would be in the linear range). Three replicate estimations were carried out for each concentration level. The specificity of the method was determined by analyzing the drug standard and test samples.