At the time of our surveys the time since clearfelling varied from 1 to 15 years. Table 1 details the date surveys were carried out. The area of clearfells

was estimated using digitized maps and varied between 0.9 and 35.2 ha. We compared the rates of native tree regeneration on these clearfelled sites Selleck Olaparib to nearby areas which had not been previously planted with conifers (control sites). We surveyed 6 control sites. The control sites were typically situated less than 1 km from the study sites. At a number of the sites former agricultural use had resulted in considerable alteration to the vegetation and the physical and chemical properties of the soil. Therefore we broadly classified all sites as either upland moorland (UM), upland improved farmland (IF) or PAWS (P) based on the present land-use of the control sites or the land-use prior to afforestation for the clearfelled sites. Both the control and the clearfelled sites were fenced to exclude stock. Capreolus capreolus (roe deer) and Cervus elaphus (red deer) were present at the Clashindarroch and Lake District sites. Only roe deer occurred selleck chemical in Bin forest. Deer control was practiced by the Forestry Commission at all sites. Sites were surveyed using 2 × 2 m temporary quadrats placed along equally spaced line transects. The

separation S   (in m) between transects and between quadrats on transects was computed by the formula ( Harmer and Morgan, 2009): S=100A/n, where A is the site area (ha) and n the number of quadrats (detailed in Table 1). Quadrats on forest track margins were omitted. In total we surveyed 1140 quadrats. Within each quadrat the species, number and height of all regenerating juveniles (defined here as either seedlings with a height ⩽50 cm or saplings with a height >50 cm) were noted. The height of saplings was measured with an extensible folding

rule. The incidence of leading stems damaged by browsing 6-phosphogluconolactonase on trees <2 m tall was noted. No attempt was made to distinguish the different birch, oak and willow spp. The distance to the nearest seed source (defined as a mature tree) was measured in the field for each tree species (all the sampled plots lay within 250 m of a native seed source). Within each quadrat we recorded the percentage of quadrat area beneath the canopy of each vascular plant species (as 2 or more species can overlap, this can result in a total vegetation cover of more than 100%) as well as the percentage cover of decaying woody debris (stumps, fallen logs and brash). Soil samples were taken from each quadrat and the pH was measured electrometrically using a soil–water paste. We were interested in the effect of brash on regeneration density so in sites that had been recently clearfelled (U6a, F2 and F4) a transect with equally spaced quadrats was oriented along a windrow and, parallel to this, another transect along the adjacent area (interrow) between the windrows.