The suitability of other biomarkers of BPA exposure such as blood has been explored; however, BPA concentrations in blood are considerably lower than those observed in urine and decrease rapidly after exposure. Hence, a large proportion of BPA in blood will be non-detectable with the current analytical methods. Additionally, even Navitoclax nmr when concentrations

are detectable, BPA concentrations in blood also vary greatly within individuals (Calafat, 2010). As in the present study, several other studies have reported differences in concentrations based on sample collection time (Calafat et al., 2005 and Mahalingaiah et al., 2008). Mahalingaiah et al. (2008) reported that urinary BPA concentrations in men and women were highest in samples collected between 1200 and 1600 h compared with concentrations in morning or late afternoon/evening samples. Teeguarden et al. showed a dramatic increase in urinary BPA concentrations following

lunch and dinner, but not breakfast, of meals containing canned foods (Carwile et al., 2011 and Teeguarden et al., 2011). Given the short half-life of BPA in humans (< 6 h (Volkel selleck products et al., 2002)), differences in exposure levels according to sample collection time may reflect sleep and dietary intake patterns (e.g., concentrations may be lower in the morning after a long period of no intake during sleep, and levels increase during the day after consuming meals contaminated with BPA or that BPA content in foods consumed later in the day is higher than that Baricitinib in foods consumed earlier in the day) (Calafat et al., 2008). Limitations of this study include imperfect data on predictor variables. For example, our questionnaire did not distinguish between canned or bottled soda consumption, with the latter

not likely to be a significant source of BPA (Lakind and Naiman, 2010). Moreover, we did not collect information on fasting time or time of last urination when we collected urine samples, both of which may impact BPA urinary concentrations (Stahlhut et al., 2009). Because we did not obtain information on time of day meals were consumed, we were not able to confirm whether higher BPA urinary concentrations observed in the afternoon/evening hours resulted from ingestion of BPA-contaminated food during the day. We also did not collect information on other potential sources of BPA exposure (e.g., dental treatment, medical devices, or exposure to thermal receipts). Furthermore, the study instruments administered were originally designed to assess exposures to pesticides rather than BPA. The food frequency questionnaire was also designed to document women’s nutrient intake during pregnancy and only limited information was gathered about food packaging. Although one question asked about consumption of canned fruit, there were no questions specifically about canned vegetables, soups, or tuna fish.