Final PCR products were subjected to electrophoresis through a 2% agarose gel and stained with ethidium bromide. Semiquantitative

RT-PCR was determined by agarose gel electrophoresis, GelDoc 2000 digitization, Scion Image Alpha 4.0.3.2. For each primer pair, assays https://www.selleckchem.com/products/PD-173074.html were designed to detect PCR product accumulation in the middle of the linear range to facilitate their relative quantification. Results Morphological characterization of rat peritoneal find more endometriosis Endometriosis was induced by transplanting endometrial tissue to the rat peritoneal wall. The endometrial explants took well to the abdominal wall and produced viable implants in 18 (90%) animals of 20. The morphological characteristics of endometriotic lesions were similar in both groups (15 and 30 days after the implantation). Most of the explants were found to be well vascularized and cystic, resembling human peritoneal endometriosis (Fig. 1A, B). Compared between groups, there was no detectable difference in size; however they were larger than the tissue fragment implanted, as shown in the measurements of the macroscopic {Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleck Anti-infection Compound Library|Selleck Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Selleckchem Anti-infection Compound Library|Selleckchem Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|Anti-infection Compound Library|Antiinfection Compound Library|buy Anti-infection Compound Library|Anti-infection Compound Library ic50|Anti-infection Compound Library price|Anti-infection Compound Library cost|Anti-infection Compound Library solubility dmso|Anti-infection Compound Library purchase|Anti-infection Compound Library manufacturer|Anti-infection Compound Library research buy|Anti-infection Compound Library order|Anti-infection Compound Library mouse|Anti-infection Compound Library chemical structure|Anti-infection Compound Library mw|Anti-infection Compound Library molecular weight|Anti-infection Compound Library datasheet|Anti-infection Compound Library supplier|Anti-infection Compound Library in vitro|Anti-infection Compound Library cell line|Anti-infection Compound Library concentration|Anti-infection Compound Library nmr|Anti-infection Compound Library in vivo|Anti-infection Compound Library clinical trial|Anti-infection Compound Library cell assay|Anti-infection Compound Library screening|Anti-infection Compound Library high throughput|buy Antiinfection Compound Library|Antiinfection Compound Library ic50|Antiinfection Compound Library price|Antiinfection Compound Library cost|Antiinfection Compound Library solubility dmso|Antiinfection Compound Library purchase|Antiinfection Compound Library manufacturer|Antiinfection Compound Library research buy|Antiinfection Compound Library order|Antiinfection Compound Library chemical structure|Antiinfection Compound Library datasheet|Antiinfection Compound Library supplier|Antiinfection Compound Library in vitro|Antiinfection Compound Library cell line|Antiinfection Compound Library concentration|Antiinfection Compound Library clinical trial|Antiinfection Compound Library cell assay|Antiinfection Compound Library screening|Antiinfection Compound Library high throughput|Anti-infection Compound high throughput screening| area (Fig. 1F). The histological characterization of endometriotic lesions revealed the presence of endometrial glands and stroma, very similar to that observed in eutopic

endometrium (Fig. 1C, D, E). We have previously observed the endometriotic lesions 90 days after the implantation and we did not detect difference in size compared with the lesions of 30 days (data not shown). Figure 1 Morphological characteristics of rat peritoneal endometriotic lesions. Lesions after 15 days (A, C) Methane monooxygenase and 30 days (B, D), eutopic endometrium (E) and histogram of implant areas (F). Most of the explants were well vascularized

(arrowheads) and cystic (arrows), resembling human peritoneal endometriosis. Compared between groups, there was no detectable difference in the lesion size. Histologically, the endometriotic tissues (C, D) were similar to the eutopic endometrium (E) because they both contained endometrial glands and stromal cells, as revealed by hematoxylin and eosin coloration. Magnification × 200. Microvessel density analysis Microvessel density was determined on the basis of vWF and αSMA-positive vessel immunodistribution. These markers were observed in the vessels located throughout the stroma, mainly around the glands. Comparison between the eutopic endometrium and the established endometriotic lesions revealed that there were more positive microvessels in the stroma around the glands in samples of endometriosis (Fig. 2). These observations were confirmed by the histomorphometry evaluation (Table 1).