Missed cleavages = 2; Fixed modifications = Carbamidomethyl (C); Variable modifications = Oxidation (M); ICPL modification at both peptide N-ter and lysine side chain. Peptide tolerance ± 1.3 Da; MS/MS tolerance ± 0.5 Da; Peptide charge = 2+ and 3+; Instrument = ESI-TRAP. Only proteins identified with a protein score above the calculated Mascot ion score, defined as the 95% confidence level, were considered. Mascot distiller was also used for protein quantification with parameters as follows: integration method: simple; correlation this website threshold: 0.8; standard error threshold: 999; Xic threshold: 0.2; max Xic width: 7; fraction threshold: 0.5 and mass time matches allowed. selleck kinase inhibitor Only peptides with an ion score above 30 were considered

for quantification. The protein ratio corresponds to the average of peptide ratios. After examination that the distribution of protein ratios was almost centered on 1, a normalization based on the median of the peptide ratios

was realized by mascot distiller on the complete dataset. Proteins with fold changes above 1.5 or below 0.66 were considered as in modified abundance. Statistical APR-246 mouse analysis All experiments were performed in triplicate, unless stated otherwise. The statistical determination of significance (α = 0.05) was calculated using a Student’s t-test on the biological replicates of each experimental condition. Acknowledgements This work was partially supported by the European Space Agency ESA/ESTEC through the PRODEX program in collaboration with the Belgian Science Policy through the BASE project. We thank Ilse Coninx, Wietse Heylen and Giuseppe Pani for excellent technical assistance. Electronic supplementary material Additional file 1: Figure S1. Morphologic analysis of a P. putida KT2440 isogenic recA mutant grown at 50 rpm and 150 rpm. Flow cytometry dot plot (forward scatter versus side scatter) of P. putida KT2440 recA mutant grown at 50

rpm (A) and 150 rpm (B). Microscopic imaging of Hoechst-stained P. putida KT2440 recA mutant grown at 50 rpm (C) and 150 rpm (D) (magnification = 1000x). ID-8 Flow cytometry histogram of P. putida KT2440 recA mutant grown at 50 rpm (grey line) and 150 rpm (black line) (E), representing the average bacterial length. (PPT 592 KB) Additional file 2: Figure S2. 3 Heat shock resistance of a P. putida KT2440 isogenic recA mutant grown at 50 and 150 rpm, as compared to wild type. Bacteria were exposed to 55°C during 30 min. (PPTX 43 KB) References 1. Wu X, Monchy S, Taghavi S, Zhu W, Ramos J, van der Lelie D: Comparative genomics and functional analysis of niche-specific adaptation in Pseudomonas putida. FEMS Microbiol Rev 2011,35(2):299–323.PubMedCrossRef 2. Dixon RA: Natural products and plant disease resistance. Nature 2001,411(6839):843–847.PubMedCrossRef 3. Manzanera M, Aranda-Olmedo I, Ramos JL, Marques S: Molecular characterization of Pseudomonas putida KT2440 rpoH gene regulation. Microbiology 2001,147(Pt 5):1323–1330.PubMed 4.