S. lycopersicum showed increased POD

activity in the presence of TYLCV. The activity of the enzyme was higher in mature than in juvenile leaves. In general, both infected and healthy leaves exhibited greater POD activity during whitefly infestation. In selleck compound the infested juvenile leaves, POD activity was much lower in the healthy leaves and increased gradually with period of exposure to B. tabaci B infestation. In contrast, the activity of the enzyme remained low in infested mature leaves in both the presence and absence of the virus even with increased exposure time. Determination of the distribution of an insect pest is critical for sampling and management. Leaf age is presumed to be associated with the within-host distribution of the geminivirus vector CHIR-99021 cost B. tabaci. Juvenile leaves will usually attract more insects due to increased nutritional value and weaker defences. Our results highlight the importance of leaf age/position on the whitefly – host plant – geminivirus interactions and have important implications for sampling and control strategies. “
“The movement protein (NSm) gene of Groundnut bud necrosis virus (GBNV) isolates from pea, mungbean, cowpea, French bean, tomato and potato collected from different locations of India were

compared to study their diversity. The NSm gene sequences of all the GBNV isolates were highly conserved and had only 0–3% diversity in amino acids and 0–10% in nucleotides. Comparison of amino acid sequence of NSm gene of 25 GBNV isolates revealed the presence of many conserved regions. Both ‘D-motif’ and ‘G-residue’, the conserved regions of ‘30K superfamily’ of virus movement protein, were present in all the isolates. Clustering of the GBNV isolates does not appear to be based on their place of origin and host plant species. “
“Plants MCE公司 of alfalfa (Medicago sativa) exhibiting general stunting, proliferation

and phyllody associated with leaf yellowing and reddening were observed in three localities of Central Serbia. Phytoplasma strains belonging to 16SrIII-B and 16SrXII-A groups were detected and identified by RFLP and sequence analysis of 16S rDNA. Stolbur phytoplasma tuf gene RFLP analysis showed the presence of the TufAY-b-type phytoplasma subgroup in 80% of symptomatic samples. This is the first report of 16SrIII-B and 16SrXII-A phytoplasma groups affecting alfalfa in Serbia. “
“Phytoplasmas were detected in Sophora japonica cv. golden and Robinia pseudoacacia with diseased branches of witches’-broom collected in Haidian district, Beijing, China. Phytoplasma cells were observed in phloem sieve elements of symptomatic S. japonica cv. golden by transmission electron microscopy. The presence of phytoplasmas was further confirmed by sequence determination of partial gene sequences of 16S rDNA, rp (ribosomal protein) and secY.